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Τετάρτη, 6 Ιουνίου 2012

Βιογραφικό Σωτήρη Νικολόπουλου



CURRICULUM  VITAE

SOTIRIS N. NIKOLOPOULOS

                                   Papastratou 79 Str.                               Aiolou 12 Str.
                                   Agrinio, 30100                                     Agia Paraskevi, 15342
                       GREECE                                              Athens, GREECE
                       Τel: +302641054611                            Tel:+302106083520
                       Fax: +302641054115                           Fax:+302106083504
e-mail: nikolops@molecular-view.com

Citizenship: Greek and US

EMPLOYMENT

March 2007-current                Founder and Scientific Executive Officer.  Center for Molecular Analysis and Research S.A., Agrinio, Greece
Sept 2001-Dec 2005               Postdoctoral Fellow (NIH, National Cancer Institute). Role of  integrins signaling in pathological angiogenesis. Cancer Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY
Aug 1998 -Aug 2001              Postdoctoral Fellow (American Heart Association, NY State Affiliate). Identification and characterization of paxillin binding proteins. Role of paxillin/integrin-linked kinase (ILK) association in mediating ILK function. Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY
Mar 1997-Mar 1998                Military service. Greek Army, Department of Health. Routine blood and urine analysis of patients’ specimens.  492 General Hospital, Alexandroupolis, Greece
Sep 1993-Dec 1996                Ph.D. Research. Department of Biological Sciences, Fordham University, Bronx, NY.  Isolation and tumor suppressor function of a novel a-actinin gene (ACTN4) from human neuroblastoma.
Sep 1993-Dec 1996                Instructor-Teaching Fellow in the laboratories of Immunology and Biology, Fordham University, Lincoln Center, NY, NY
Sep 1990-May 1993                Research Associate. Department of Biological Sciences, Fordham University, Bronx, NY.  Mechanisms of action of immunomodulators (mainly TNF-a) and their efficacy as anticancer agents.
Sep 1990-May 1993                Instructor-Teaching Fellow in the laboratory of Biology, Fordham University, Bronx, NY
July 1989- July 1990               Laboratory Technologist II.  Molecular analysis of the meningioma locus. Division of Molecular Genetics, Department of Research, North Shore University Hospital, Cornell University Medical College, Manhasset, NY
Mar 1988- May 1989              Internship. Pulsed-field gel electrophoresis as a method of detection of mutations in human tumors.  Laboratory of Molecular Genetics, Department of Research, North Shore University Hospital, Cornell University Medical College, Manhasset, NY
Sep 1987- Dec 1988               Graduate Assistant in the Laboratories of Medical Microbiology and Immunology, Department of Health Sciences, Long Island University, Brookville, NY

EDUCATION

Sep 1990- Dec 1996   PhD in Molecular and Cellular Biology, Fordham University, Bronx, NY, USA
Sep 1987- May 1989   MS in Medical Biology (Immunology), Long Island University, Brookville, NY, USA
Sep 1982- May 1987   BS in Biology, National and Kapodistrian University of Athens, Athens, Greece


HONORS/AWARDS           
·         NIH Fellowship, Ruth L. Kirschstein National Research Service Award (July 2003-July 2006)
·         American Heart Association Fellowship, NY State Affiliate (July 1999 - June 2002)         
·         NIH travel award for Keystone Symposia 2000 (March, 2000)
·         Postdoctoral grant, intramural SUNY (October 1998 - October 1999)
·         Certificate of Merit for outstanding performance, dedication and effort
in achieving the goals of North Shore University Hospital (April, 1990)
·         Dean’s award for academic excellence in graduate studies, School of Health Professions, Long Island University (May, 1989)
TECHNICAL EXPERTISE

Biochemistry - Cell Biology - Molecular Biology – Bioinformatics - Immunology - Virology – Genetics

Establishment and culture of primary cell lines from tissues – Transfection of primary cells - Cell sorting - Isolation and manipulation of nucleic acids and proteins from cells and tissues - Southern, Northern, and Western blotting - Pulsed-field gel electrophoresis - Construction and screening (radioactively or immunologically) of genomic and cDNA libraries (included subtracted ones) - Molecular cloning - PCR, RT-PCR - Sequencing - Gel shift assays - Nuclear run on assays - Eukaryotic transfections and cellcloning - Soft agar assays - Cytogenetic techniques – Immunocytochemistry – Immunochemical techniques (Immunoprecipitation, ELISA, FACS, Immunofluorescence) - F.I.S.H. - Metabolic Isotopic Labeling - Mammalian cell based assays on various substrates (cell adhesion, spreading, migration, proliferation, apoptosis) - Electron microscopy - DNA microarray technology – Virus work – Construction of viral constructs - Viral cell infection and processing –  Gene therapy Mouse work - Gene targeting - Mouse husbandry - Genetic crossing - genotyping –  Angiogenesis and tumorigenesis assays in vitro and in vivo – Matrigel invasion assay –
Retinal response to hypoxia assay - Wound healing in vivo - Mouse surgery and transplantation

PUBLICATIONS

Turner CE, Brown MC, Perrota JA, Riedy MC, Nikolopoulos SN, McDonald RA, Bagrodia S, Thomas S, Leventhal PS (1999). Paxillin LD4 motif binds PAK and PIX through a novel 95kDA ankyrin-repeat, ARF-GAP protein: a role in cytoskeletal remodeling. Journal of Cell Biology 145:851-863.

Nikolopoulos SN, Spengler BA, Kisselbach K, Evans AE, Biedler JL, Ross RA (2000). The human non-muscle alpha-actinin protein encoded by the ACTN4 gene suppresses tumorigenicity of human neuroblastoma cells. Oncogene 19:380-386.

Scammell JG, Reddy S, Valentine DL, Coker TN, Nikolopoulos SN, Ross RA (2000). Isolation and characterization of the human secretogranin II gene promoter. Molecular Brain Research 75:8-15.

Nikolopoulos SN, Turner CE (2000). Actopaxin: A new focal adhesion protein that binds paxillin LD motifs and actin and modulates cell adhesion. Journal of Cell Biology 145:851-863.

Nikolopoulos SN, Turner CE (2001). Integrin-Linked Kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions. Journal of Biological Chemistry 276:23499-23505.

West KA, Zhang H, Brown MC, Nikolopoulos SN, Horwitz AF, Turner CE (2001). The LD4 motif of paxillin regulates cell spreading and motility through an interaction with PKL. Journal of Cell Biology 154:161-176. 

Nikolopoulos SN, Turner CE (2002). Molecular dissection of Actopaxin-Integrin-Linked Kinase-Paxillin interactions and their role in subcellular localization. Journal of Biological Chemistry  277:1568-1575.

Curtis MS, Nikolopoulos SN, Turner CE (2002). Actopaxin is phosphorylated during mitosis and it is a substrate for CDK1. Biochemical Journal  363:233-242.

Nikolopoulos SN, Blaikie P, Yoshioka T, Guo W, Giancotti FG (2004).  Integrin b4 signaling promotes tumor angiogenesis. Cancer Cell 6:471-483.

Nikolopoulos SN, Giancotti FG (2005).  Netrin-Integrin Signaling in Epithelial Morphogenesis, Axon Guidance, and Vascular Patterning. Cell Cycle 4(3):429-433.

Nikolopoulos SN, Blaikie P, Yoshioka T, Guo W, Puri C, Tacchetti C, Giancotti FG (2005). Targeted deletion of the integrin b4 signaling domain suppresses laminin-5-dependent nuclear entry of MAP kinases and NF-kB causing defects in epidermal growth and migration. Molecular and Cellular Biology 14: 6090-6102.

Roberts JE, Nikolopoulos SN, Oktem O, Giancotti F, Oktay K. (2009). Integrin beta-4 signaling plays a key role in mouse embryogenesis  Reproductive Science 16(3):286-93.

Petridis AK, Nikolopoulos SN, El-Maarouf A. (2011). Physical and functional cooperation of neural cell adhesion molecule and β1-integrin in neurite outgrowth induction. J Clin Neurosci. 2011 Aug;18(8):1109-13. Epub 2011 Jun 29.

INVITED TALKS

Nikolopoulos SN. Regulation of tumor angiogenesis and epidermal growth by the integrin β4 signaling domain. Invited talk. IMBB Forth, Heraklion, Crete, Greece, January 2007.

Nikolopoulos SN. Regulation of tumor angiogenesis and epidermal growth by the integrin β4 signaling domain. Invited talk. Academy of Athens, Athens, Greece, July 2006.

Jeffrey E. Roberts, Sotiris N. Nikolopoulos, Ozgur Oktem, Zev Rosenwaks, Filippo Giancotti, Kutluk Oktay. Integrin Beta-4 Signaling Plays A Key Role In Mouse Embryogenesis. The Society for Reproductive Endocrinology and INF, October 2005.

Nikolopoulos SN, Blaikie P, Yoshioka T, Giancotti FG. Integrin b4 signaling promotes tumor angiogenesis. Cold Spring Harbor  Symposia (Protein Phosphorylation and Cell Signaling), May 2005.

Giancotti FG, Nikolopoulos SN, Blaikie P, Guo W, Yoshioka T.  Genetic analysis of integrin a6b4 signalling. Keystone Symposia, March 2003.

Nikolopoulos SN, Blaikie P, Yoshioka T, Giancotti FG.  Role of a6b4 integrin in postnatal and pathological angiogenesis. Gordon Research Conferences (Signaling by Adhesion Receptors), July 2002.

Nikolopoulos SN, Turner CE. Identification and characterization of a novel 42 kDa actin- and paxillin-binding protein localized in focal adhesions.  Keystone Symposia, March 2000.

West KA, Brown, MC, Nikolopoulos SN, Turner CE. The LD4 domain of paxillin functions in the regulation of cell adhesion, spreading and motility in CHO.K1 fibroblasts. SUNY Upstate Medical University Program in Cell and Molecular Biology Retreat by the Lake Poster Session, June 2000.

West KA, Brown MC, Nikolopoulos SN, Turner CE. The LD4 domain of paxillin functions in the regulation of cell spreading in CHO.K1 fibroblasts.  Keystone Symposia, March 2000.

Ross RA, Dugliss JW, Nikolopoulos SN, Spengler BA, Biedler JL. Differential expression and regulation of the RE1-silencing transcription factor (REST) in human neuroblastoma cell variants. Proc Annu Meet Am Assoc Cancer Res 1997;38:A1029.

Nikolopoulos SN, Spengler BA, Biedler JL, Ross RA. Isolation, characterization, and tumor suppressor function of a novel a-actinin gene from human neuroblastoma.  Proc Annu Meet Am Assoc Cancer Res 1996;37:A3489.

Spengler BA, Nikolopoulos SN, Biedler JL, Ross RA. P98 a novel protein immunologically related to N-myc characterizes human neuroblastoma S-type cells.  Proc Annu Meet Am Assoc Cancer Res 1994

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